Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • #16
    setting -m 2 is processing many more blocks. However, even at m=5 it was near-instant.

    I noticed a section "What happens when my sample is a mixture, or diploid?" in the Quiver FAQ

    Does this mean accuracy is suspect also for genomes with transposable elements?

    Comment


    • #17
      Quiver is jumping over the regions with inadequate effective coverage [== coverage after MapQV filter]. My understanding was you had close to 30x coverage?

      You can try --minMapQV=0 --minCoverage=0 to completely disable input filtering, try it.
      MapQV is effectively quantized at either 0 or 255 in my experience.

      Comment


      • #18
        yes, according to MIRA's mapping assembly (after pacbio error-correction), I have 30x coverage. There are a couple of problem regions of course (chiefly involving the transposons)

        Comment


        • #19
          Well, try turning off the mapQV filter and see what happens. The remaining explanation is that your draft assembly contigs are either redundant or ridiculously repetitive, so there is no confidence in the mapping.

          Comment


          • #20
            pag, if you are still having difficulties I can get you in touch with our tech support. Let me know.

            Comment

            Latest Articles

            Collapse

            • seqadmin
              Understanding Genetic Influence on Infectious Disease
              by seqadmin




              During the COVID-19 pandemic, scientists observed that while some individuals experienced severe illness when infected with SARS-CoV-2, others were barely affected. These disparities left researchers and clinicians wondering what causes the wide variations in response to viral infections and what role genetics plays.

              Jean-Laurent Casanova, M.D., Ph.D., Professor at Rockefeller University, is a leading expert in this crossover between genetics and infectious...
              Today, 10:59 AM
            • seqadmin
              Addressing Off-Target Effects in CRISPR Technologies
              by seqadmin






              The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...
              08-27-2024, 04:44 AM

            ad_right_rmr

            Collapse

            News

            Collapse

            Topics Statistics Last Post
            Started by seqadmin, 09-06-2024, 08:02 AM
            0 responses
            13 views
            0 likes
            Last Post seqadmin  
            Started by seqadmin, 09-03-2024, 08:30 AM
            0 responses
            18 views
            0 likes
            Last Post seqadmin  
            Started by seqadmin, 08-27-2024, 04:40 AM
            0 responses
            25 views
            0 likes
            Last Post seqadmin  
            Started by seqadmin, 08-22-2024, 05:00 AM
            0 responses
            387 views
            0 likes
            Last Post seqadmin  
            Working...
            X