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  • Stefania_IT
    Junior Member
    • Jun 2013
    • 3

    #1

    HTSeq, GTF vitis vinifera, mismatched quotes

    Hi!
    I'm trying using HTSeq.
    I have a problem with thr GTF file.
    I'm working with vitis vinifera and i downloaded the gtf file from the website plant.ensembl.

    I have this kind of error.
    I typed this command:
    htseq-count --mode=intersection-nonempty /Users/Desktop/accepted_hits.sam /Users/Desktop/Vitis_vinifera.IGGP_12x.18.gtf > gene_count.txt

    and i got this message:

    Error occured in line 5484 of file /Users/Desktop/Vitis_vinifera.IGGP_12x.18.gtf.
    Error: The attribute string seems to contain mismatched quotes.
    [Exception type: ValueError, raised in __init__.py:174]



    Does someone know something about how to fix this?
    Does someone know another site for downloading the GTF for vitis vinifera?

    Thank you so much!
  • Simon Anders
    Senior Member
    • Feb 2010
    • 995

    #2
    Plant people seem to have a habit of using strange characters in their gene names, such as semicolons and apostrophes, and HTSeq's GFF parser sometimes stumbles over these. Eventually, I have to make the parser more robust, but until then, you can maybe just try to find and remove these charcters.

    Comment

    • Stefania_IT
      Junior Member
      • Jun 2013
      • 3

      #3
      Did it!
      I found them and changed with an underscore.
      Now is running!
      Thank you.

      Comment

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