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  • Kennels
    Senior Member
    • Feb 2011
    • 149

    #1

    does accepted_hits or unmapped .bams from tophat2 include reads mapping to junctions?

    Hi

    Does anyone know if the 'accepted_hits.bam' file from the tophat2 output include reads that were mapped to junctions? I did not use the 'fusion-search' option.
    If so, what tags are used in the .bam file to identify these? I can't find relevant information in the tophat page.
    Or are these included in the unmapped.bam file?

    thanks
  • kopi-o
    Senior Member
    • Feb 2008
    • 319

    #2
    Yes, the accepted_hits.bam file includes them. Look for 'N' characters in the CIGAR string (6th column).

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