Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • cummeRbund diffData() or fpkm() values

    I'm getting different FPKM values from diffData() and fpkm() on a couple of different isoform IDs

    Code:
    > diffData(getFeatures(cuff,"TCONS_00246566"))
          isoform_id   sample_1 sample_2  status value_1 value_2 log2_fold_change test_stat p_value q_value significant
    1 TCONS_00246566 macrophage monocyte LOWDATA       0       0    -1.79769e+308         0       1       1          no
    diffData() reports an fpkm of 0 for sample_2 (reported as "value_2")

    Code:
    > fpkm(getFeatures(cuff,"TCONS_00246566"))
          isoform_id sample_name      fpkm conf_hi conf_lo quant_status    stdev
    1 TCONS_00246566  macrophage 0.0000000  0.0000       0      LOWDATA  0.00000
    2 TCONS_00246566    monocyte 0.0972739 49.0851       0      LOWDATA 24.49391
    but fpkm() reports an fpkm of ~0.09 for sample_2

    I know that csHeatmap() uses fpkm() to extract values for plotting, but which is the most accurate value?

    I thought cuffdiff values (available from diffData()) were supposed to be the most accurate?

Latest Articles

Collapse

  • seqadmin
    Recent Advances in Sequencing Analysis Tools
    by seqadmin


    The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
    05-06-2024, 07:48 AM
  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 07:03 AM
0 responses
15 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-10-2024, 06:35 AM
0 responses
37 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-09-2024, 02:46 PM
0 responses
43 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-07-2024, 06:57 AM
0 responses
38 views
0 likes
Last Post seqadmin  
Working...
X