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  • pari_89
    Member
    • Apr 2013
    • 55
    #1

    Assembling against a reference

    Hello everyone,

    I have been doing some reading about mapping and assembling against a reference and I cannot quite understand the difference? What I understand it that mapping against a reference gives alignment scores while assembling produce a set of contigs.

    I have used CLCworkbench - maps reads against a reference, runMapping from Newbler and MIra to do a mapping assembly? I get a list of contigs as well.

    Some sources say that mapping is not assembly. Can anyone please explain to me or give me some papers to understand this please.

    Thank you.
    Tags: None
  • swaraj
    Member
    • Feb 2012
    • 50
    #2
    This should help.

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    http://www.ncbi.nlm.nih.gov/pubmed/21897427

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