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  • jeffseq
    Junior Member
    • Mar 2013
    • 9

    #1

    VCFtools vcf-consensus error

    vcf-consensus fails to pick any variants in VCF file involing last position:

    e.g. we have:

    ref.fa:
    >ref
    ATCG

    variants in ref.vcf:

    ref 1 . A T . . .
    ref 4 . G T . . .

    when we do: cat ref.fa |vcf-consensus ref.vcf.gz
    get:

    >ref
    TTCG

    position 1 is OK but position 4, G->T is ignored.

    Also, if we have:

    ref 2 . TCG T . . .

    TCG-> T deletion is also ignored. because G is the last position?

    Any other good alternative tools to use (besides GATK, which I have other issues with it too)?
  • chuanchao
    Junior Member
    • Aug 2013
    • 3

    #2
    when I do: cat ref.fa |vcf-consensus ref.vcf.gz:

    The fasta sequence does not match the REF at Y:2649856. g(cactta) in .fa, G in .vcf, frz=0

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