Dear all,
I have an experiment where I test three different conditions (you know, usual stuff ). Total RNA was extracted and sequenced by RNAseq, later determined gene expression for each condition, and so on...
Differential gene expression was made with bowtie,tophat,cufflinks,cuffdiff... and..
...One of my transcripts overexpressed was: ¡DICER! (a ribonuclease known to process pre-miRNAs and dsRNAs). I really suspect that my data from RNAseq has transcripts which are these non coding RNA, and I want to retrieve these sequences
The hard thing: How?
thx
I have an experiment where I test three different conditions (you know, usual stuff ). Total RNA was extracted and sequenced by RNAseq, later determined gene expression for each condition, and so on...
Differential gene expression was made with bowtie,tophat,cufflinks,cuffdiff... and..
...One of my transcripts overexpressed was: ¡DICER! (a ribonuclease known to process pre-miRNAs and dsRNAs). I really suspect that my data from RNAseq has transcripts which are these non coding RNA, and I want to retrieve these sequences
The hard thing: How?
thx
Comment