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  • CASAVA combine samples across all lanes

    By default, when demultiplexing with CASAVA, same sample across multiple lanes is added to separate FASTQ file for each lane. Is there a way to have one FASTQ file for each sample?

    There is a --fastq-cluster-count option so that FASTQs are not limited to a certain amount of reads. Maybe there is something to make FASTQs not limited to one lane?

  • #2
    Nope, this is not possible with CASAVA. You'll have to settle for taking the per lane FASTQ from CASAVA and concatenating them after the fact.

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    • #3
      which shouldn't be a problem since you can use cat on every unix OS for that...

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