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  • volavii
    Member
    • Apr 2013
    • 16

    #1

    which assembler shoud i use after mapping with BWA

    Hey

    i want to assemble my reference mapped reads (BWA2) into a consesus sequence...
    Is there a programm or do i have to write a script?
  • krobison
    Senior Member
    • Nov 2007
    • 734

    #2
    Second Google hit for "consensus sequence from bam"



    which gives this command

    samtools mpileup -uf reference.fa alignment.bam | bcftools view -cg - | vcfutils vcf2fq

    (requires samtools, vcftools, vcftools)

    Comment

    • volavii
      Member
      • Apr 2013
      • 16

      #3
      thanks krobison

      Comment

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