Hello All,
I have RPKM values for two treatment conditions (lets say control and diseased). Each treatment has 3 replicates. I have transformed all the replicate data like so: [log2(count+1)]. Now if I wanted to get a single value of gene expression for control and diseased, what do I need to do? Average, Median or sum of replicates.
I read a number of papers where data transformation is discussed (log2(count+1)) but could not find references on how to obtain a single value quantifying the gene expression in control and diseased.
The ultimate goal of course is differential gene expression. For this, is the above at all necessary?
Any help will be very beneficial.
Thanks
I have RPKM values for two treatment conditions (lets say control and diseased). Each treatment has 3 replicates. I have transformed all the replicate data like so: [log2(count+1)]. Now if I wanted to get a single value of gene expression for control and diseased, what do I need to do? Average, Median or sum of replicates.
I read a number of papers where data transformation is discussed (log2(count+1)) but could not find references on how to obtain a single value quantifying the gene expression in control and diseased.
The ultimate goal of course is differential gene expression. For this, is the above at all necessary?
Any help will be very beneficial.
Thanks
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