Hi,
I am running tophat and cufflink of dog RNA-seq data using human reference genome. I got very few genes. I got a lot more genes using canine reference genome.
I know I will probably have to relax some parameters in alignment step etc.
has anyone here done this before? Could you please give me some advice?
thanks
I am running tophat and cufflink of dog RNA-seq data using human reference genome. I got very few genes. I got a lot more genes using canine reference genome.
I know I will probably have to relax some parameters in alignment step etc.
has anyone here done this before? Could you please give me some advice?
thanks
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