Is there any free tool similar to Blast2go?

[Hel]

Hi all,
Is there any free tool similar to Blast2go?
I've just started my PhD on NGS, in a non model species of insects (belonging to Coleopterans). I've just received a large amount of sequences from 454 FLX to annotate. I've read about Blast2go, but I would like to explore any other available tools , if any.
Thanks you!

[maubp]

The Blast2GO team have never to my knowledge ever stated what the software licence, so it is not "free" as in open (i.e. it is not open source software):

https://groups.google.com/forum/#!topic/blast2go/PAFUJ9vQDgE

Blast2GO does now offer a paid for "Pro" service, but the basic version is still "free" as in money. And if your group has the technical capabilities, this can be used at a large scale on local infrastructure.

(Note normally you wouldn't run Blast2GO on raw reads anyway, try to assemble them first.)

If you do have a large set of sequences I would run the BLAST search against the NCBI NR database outside of Blast2GO using the standalone NCBI BLAST+ tools, and then give that to Blast2GO. It is also very worthwhile to setup a local Blast2GO database which is far faster than the (free) server provided in Spain.

We do this local on our computer cluster from within Galaxy, see: http://toolshed.g2.bx.psu.edu/view/peterjc/blast2go

[uloeber]

GOblet

Hi Hel,
you can use GOblet. http://journal.imbio.de/article.php?aid=104

[maubp]

Hi Hel,
you can use GOblet. http://journal.imbio.de/article.php?aid=104

According to the download http://goblet.molgen.mpg.de/goblet.zip the Goblet license is "GPL Version 3 unless otherwise noticed in the directories" so this is free and open source

[Hel]

Thanks! I will explore these options