Splicing Analysis in Partek RNA-Seq workflow

[xguo]

Hi, there,

Does anyone know how Partek calculates Alternative Splcing p-value in their RNA-Seq workflow? It seems that they use an EM algorithm to deduce expression of individual transcripts based on the paper in Nucleic Acids Res. 2006 Jun 6;34(10):3150-60. Differential expression p-value is shown for each transcript, but a single alternative splicing p-value is presented for each gene.

thanks
Xiang

[RNA-seq]

Partek has a white paper explaining how to calculate alternative splicing p-value on the tutorial webpage. Open the software, go to "help"--"online tutorials"--looking for "white papers" tab.