I got the above error when trying to run MACS 1.4.2 (I'm copying the parameter/output below). I ran previously the same sample/control with mfiold=32 but got no peaks. Than changed mfold to 10 and got this error. Does anyone know what is going on and what to do about it?
MACS input/output (error message is at the end):
========================================================
effective genome size = 1.00e+09
tag size = 40
band width = 300
model fold = 10
pvalue cutoff = 1.00e-05
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL181494.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL182298.1, plus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL180583.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL183373.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL192011.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL185054.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL178673.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL187281.1, plus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL181933.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL189987.1, plus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL185212.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL186088.1, plus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL184880.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL189261.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL190030.1, plus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL181273.1, plus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL191240.1, plus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL190257.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL176051.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL183262.1, minus strand!
....
and plenty of similar warnings about missing chromosomes
....
INFO @ Sat, 13 Jul 2013 04:29:31: #1.2 read input tags...
Traceback (most recent call last):
File "/home/g2main/linux3.2-x86_64/bin/macs", line 273, in
main()
File "/home/g2main/linux3.2-x86_64/bin/macs", line 57, in main
(treat, control) = load_tag_files_options (options)
File "/home/g2main/linux3.2-x86_64/bin/macs", line 256, in load_tag_files_options
control = options.build(open2(options.cfile, gzip_flag=options.gzip_flag))
File "/home/g2main/linux3.2-x86_64/lib/python2.7/MACS/IO/__init__.py", line 1480, in build_fwtrack
(chromosome,fpos,strand) = self.__fw_parse_line(thisline)
File "/home/g2main/linux3.2-x86_64/lib/python2.7/MACS/IO/__init__.py", line 1500, in __fw_parse_line
bwflag = int(thisfields[1])
ValueError: invalid literal for int() with base 10: '1:N:0:'
MACS input/output (error message is at the end):
========================================================
effective genome size = 1.00e+09
tag size = 40
band width = 300
model fold = 10
pvalue cutoff = 1.00e-05
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL181494.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL182298.1, plus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL180583.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL183373.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL192011.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL185054.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL178673.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL187281.1, plus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL181933.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL189987.1, plus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL185212.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL186088.1, plus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL184880.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL189261.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL190030.1, plus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL181273.1, plus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL191240.1, plus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL190257.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL176051.1, minus strand!
WARNING @ Sat, 13 Jul 2013 04:29:05: NO records for chromosome GL183262.1, minus strand!
....
and plenty of similar warnings about missing chromosomes
....
INFO @ Sat, 13 Jul 2013 04:29:31: #1.2 read input tags...
Traceback (most recent call last):
File "/home/g2main/linux3.2-x86_64/bin/macs", line 273, in
main()
File "/home/g2main/linux3.2-x86_64/bin/macs", line 57, in main
(treat, control) = load_tag_files_options (options)
File "/home/g2main/linux3.2-x86_64/bin/macs", line 256, in load_tag_files_options
control = options.build(open2(options.cfile, gzip_flag=options.gzip_flag))
File "/home/g2main/linux3.2-x86_64/lib/python2.7/MACS/IO/__init__.py", line 1480, in build_fwtrack
(chromosome,fpos,strand) = self.__fw_parse_line(thisline)
File "/home/g2main/linux3.2-x86_64/lib/python2.7/MACS/IO/__init__.py", line 1500, in __fw_parse_line
bwflag = int(thisfields[1])
ValueError: invalid literal for int() with base 10: '1:N:0:'