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  • BhariD
    Member
    • Mar 2013
    • 15

    over-represented mitochondrial sequences in the RNA-seq data

    I have Illumina RNA-seq data. I have been finding a lot of overrepresented sequences in the library (>0.1% of total, some are even 0.3%) in all the samples I have.

    These over-represented sequences are 50bp long and do not have any hits to known adapter/primer sequences.

    Few of these over-represented sequences are simply string of polyA's (which can be trimmed easily), but majority of them have hits to human mitochondrial genome (this is based on the BLAST nt similarity search), for example, "CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT".

    I am struggling on how to process or clean-up this raw data before doing any mapping or downstream analyses. I guess my question is:

    1) Is this a result of contamination? If so, how should one proceed, I mean should I trim reads with these sequences or remove the reads with these sequences completely or leave them in there? what is the best approach?

    2) Could these be biologically significant sequences? just happen to be similar to mitochondrial DNA. How would I know that? Is there a way to measure this?

    I would really appreciate your help or any suggestions you might have.

    Thank you!
  • sara_ahmed
    Member
    • Nov 2011
    • 13

    #2
    Was this prepared using polyA enrichment? If so, certain mitochondrial transcripts have polyA tails and will show up in your data. You might be able to look up whether the sequences you are finding in your data correspond to mitochondrial transcripts that are known to be polyadenylated.

    Sara Ahmed, PhD | Director of Sequencing | Cofactor Genomics
    3141 Olive St. | St. Louis, MO 63103 | tel. (314) 531-4647

    Comment

    • NextGenSeq
      Senior Member
      • Apr 2009
      • 482

      #3
      mtRNA is among the most abundant transcripts in total RNA and yes mtRNA is biologically significant. Some geneticists primarily study mt mutations and their contributions to disease.

      Comment

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