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I test for differential expression genes, where I have 6 biological replicates in one condition and 18 in the other.
When I use the "estimateDispersions" , it gives me the following error:
>cds <- estimateSizeFactors(countdata)
>cds <- estimateDispersions(cds)
Warning:
In parametricDispersionFit(means, disps) : Dispersion fit did not converge.
Do you know why this warning arise?
When I use the "estimateDispersions" , it gives me the following error:
>cds <- estimateSizeFactors(countdata)
>cds <- estimateDispersions(cds)
Warning:
In parametricDispersionFit(means, disps) : Dispersion fit did not converge.
Do you know why this warning arise?