What is a parsimonious way to determine if two bam files are from the same individual? Is there an off-the shelf, open source implementation?
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I'd just run them through the same genotyper and then compare the genotypes with vcf-compare in vcf-tools. Or run it through a somatic detection program like VarScan2, and expect to see very few differences output. -
I've done this before with array data as validation for SNPs; when looking at SNP calls on the array spots, I would always get well over 90% concordance between calls if the same person was sequenced in multiple libraries. Between different people I would never get over 50% concordance, and this is just looking at relatively common SNPs on the array. So if you call SNPs and look at ones with at least a quality score of say 30 and coverage of 10x (by mpileup), it should be super obvious.Comment
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The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.Today, 06:37 PM -
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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