Hi everybody, i'm confussed about how can i verify if the information obtained from a MiSeq Illumina sequencing of a transcriptome is robust or not.
I have a sequencing of three tissues obtaining a total of 29.603.685 reads of 151 bp lenght, taht where filtered and trimmed, and an assembly of the high quality reads, obtaining a total of 64.924 transcripts with a total of 53.409.526 bp. I need to know if the number of reads used are enough to verify if the information is robust or not.
Could you help me with that? Please help me with a reference too!
Best regards!
I have a sequencing of three tissues obtaining a total of 29.603.685 reads of 151 bp lenght, taht where filtered and trimmed, and an assembly of the high quality reads, obtaining a total of 64.924 transcripts with a total of 53.409.526 bp. I need to know if the number of reads used are enough to verify if the information is robust or not.
Could you help me with that? Please help me with a reference too!
Best regards!
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