MiSeq Illumina output

[mruizm]

Hi everybody, i'm confussed about how can i verify if the information obtained from a MiSeq Illumina sequencing of a transcriptome is robust or not.
I have a sequencing of three tissues obtaining a total of 29.603.685 reads of 151 bp lenght, taht where filtered and trimmed, and an assembly of the high quality reads, obtaining a total of 64.924 transcripts with a total of 53.409.526 bp. I need to know if the number of reads used are enough to verify if the information is robust or not.

Could you help me with that? Please help me with a reference too!

Best regards!

[GenoMax]

Is there a reference genome (or one from a close relative) available? Do you have an idea of what the expected genome size is?

[mruizm]

There is no reference genome, is a de novo analysis. And i don't know if there is a genome size expected because is a transcriptome analysis!