Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Melissa
    Senior Member
    • Aug 2008
    • 124

    #1

    Scaffolding problem with Velvet

    Hi,

    Does anyone has problem with scaffolding using velvet? The contigs I generated are small and do not contain Ns.

    I'm sure I follow the manual:
    -First I merge the paired end reads using shufflesequences.pl
    -./velveth for shortPaired
    -./velvetg for paired-ends by giving insert size and expected coverage

    What went wrong?
  • avt
    Junior Member
    • Dec 2009
    • 3

    #2
    try some large exp_cov, and how did you run Velveth
    Can you tell me how big is your data? I often fail to run it on my (big) file..!
    Last edited by avt; 01-05-2010, 10:18 AM.

    Comment

    • Melissa
      Senior Member
      • Aug 2008
      • 124

      #3
      My data is around1.3Gb.

      Comment

      Latest Articles

      Collapse

      • kinpict.com
        Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by kinpict.com
        The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
        Today, 06:37 PM
      • SEQadmin2
        16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by SEQadmin2





        NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
        10-30-2026, 06:46 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 11-12-2026, 07:59 AM
      0 responses
      27 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 11-05-2026, 09:25 AM
      0 responses
      35 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 11-03-2026, 11:54 AM
      2 responses
      56 views
      0 reactions
      Last Post kinpict.com  
      Started by SEQadmin2, 10-28-2026, 09:57 AM
      0 responses
      175 views
      0 reactions
      Last Post SEQadmin2  
      Working...