Cufflinks - twofold increase in expression

[cement_head]

How does one judge using the (RPKM values) what is a twofold change in expression?

For example, for a given gene, a value of 1.0 is background. Would a value of 3.0 be equivalent to a two fold increase of expression?

Thanks,

[sdriscoll]

Rpkm values are interpreted as they are. A value of 10 is 2x a value of 5 and is therefore two-fold increased. I'm not sure what you're asking here.

[cement_head]

Rpkm values are interpreted as they are. A value of 10 is 2x a value of 5 and is therefore two-fold increased. I'm not sure what you're asking here.

That's excatly what I'm asking; thank you!