Tweet
Hi there!
Like the title says, How do i get those reads, that are not fully aligned in BLAST?
Someone already tried it?
Like the title says, How do i get those reads, that are not fully aligned in BLAST?
Someone already tried it?
-
-
What do you mean by fully aligned?savetherhino.org -
If you aligned reads against a database, you will have to extract the identifiers of reads that did align to something first and then extract those reads from the original input file. There is no way to fully recover incompletely aligned query sequences from blast output (as far as I am aware).Comment
-
I've been doing something similar recently - mainly via awk command from a tabbed blast+ output and then using this file to filter an input sequence file.
If you elaborate a little on what you are trying to do, I may be able to help.Comment
-
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM
Comment