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  • wilson90
    Member
    • May 2012
    • 48

    #1

    Cuffdiff and Cufflink Error with -g option?

    Dear all,

    I am using cufflink to produce transcript abundance level estimation, and cuffdiff to do differential analysis.

    However, I have come across this contradiction that certain genes, although annotation has been given, is reported as "new" under -g option.

    Example,
    Entry 1.
    XLOC_001015 - - XLOC_001015 Tnnt2 TSS1531,TSS1532,TSS1533,TSS1534 chr1:137732962-137748850 [FPKM values ..]

    Entry 2.
    XLOC_002303 - - XLOC_002303 - TSS3895 chr1:137732962-137748850 [FPKM values ..]

    Quite obvious that these 2 lines in the cuffdiff (genes.fpkm_tracking) file are describing the same gene?

    Any plausible explanation?

    Thank you.

    Best Regards,
    Wilson

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