Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Kennels
    Senior Member
    • Feb 2011
    • 149

    #1

    tgicl/cap3 segmentation fault on just one asm directory

    Hi,

    This has been cross-posted at the TGICL sourceforge page (https://sourceforge.net/p/tgicl/support-requests/3/)

    I'm using TGICL clustering pipeline, but I'm really stuck in the assembly (cap3 step) of just one cluster which is holding up my whole project, and I'm hoping someone has seen this before and can provide a solution.

    I have no problem with the clustering phase, but I'm running into a segmentation fault for one of my asm directories during assembly.

    I've installed the 64-bit, Intel version of CAP3 as part of the TGICL pipeline. My server is running on Intel Xeon processors (SUSE Linux Enterprise Server), and I can allocate up to 256 Gb of memory per node, but I don't think memory limitation is the problem.

    Using 16 CPUS, all but one assembly directory have assembled ok. In the asm_1 directory that runs into the error, this is what I get:

    ####
    sh: line 1: 63326 Segmentation fault cap3 CL1 -p 93 > CL1.align
    Error! cap3 failure detected (code=35584) on: CL1
    ####

    It seg faults almost immediately when cap3 tries to assemble this cluster (other asm dirs are ok).
    I have tried it independently (cap3 standalone) on just this directory as well, with the same error.

    In contrast, I have another assembly which runs to completion just fine. The only difference is the size of the cluster.

    Failed cluster:
    1,359,975,661 bytes (1.35Gb)
    962,144 sequences

    Successful cluster:
    1,135,345,720 bytes (1.1Gb)
    731,031 sequences

    The successful cluster ran on a 64Gb node, but the failed cluster gave seg fault even on 256Gb node. As an aside, I did try the 64bit opteron version of cap3, and it actually started running but died on a memory error after about 60 hours (this happened even on 256Gb node).
    ####
    Ran out of memory: 52067036860 bytes requested.
    Error! cap3 failure detected (code=256) on: CL1
    ####

    So I think it's something in the cap3 (intel, 64bit) code, that is not allocating memory correctly when input is above certain size.

    I might have to contact cap3 author to address this problem, but has anyone else encountered this and came up with a solution? Any suggestions/comments greatly appreciated.

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
52 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
39 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
46 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
46 views
0 reactions
Last Post SEQadmin2  
Working...