Oh thanks!
My fasta file was in a directory which required permission for writing onto.
I was running the command from a separate directory and assumed the new file would be created in this directory instead of the one that contained my .fasta file. Solves my problem.
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Have you checked the permissions for the directory where the .fai file is going to be written, and also, that there is enough space on the computer to write the .fai file?Leave a comment:
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creating .fai from .fa file using samtools faidx command
Hi everyone,
I am trying to create a FASTA index from a FASTA file using samtools:
./samtools faidx /home/krathi/data/ChIPseq/mm9/mm9.fa
but it shows me the error:
[fai_build] fail to write FASTA index /home/krathi/data/ChIPseq/mm9/mm9.fa.fai
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The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...11-06-2024, 07:24 PM -
Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...10-18-2024, 07:11 AM
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