Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Mosaik Aligning with 454 Reads

    How long will it take to finish the Mosaik Aligning work?

    In the manual for Mosaik1.0, MosaikAligner aligned the lane of 6,563,762 Illumina paired-end reads(36bp) in 45.9minutes (2,390 paired-end reads/s) to the entire human genome(2.9Gbp) using 8processor cores.

    And in the manual for Mosaik, Aligner setting for 454 GS-FLX : -hs 15 -mm 0.05 -act 26

    But in the case of version 0.9.891, the lane of 119,263 '454 reads'
    expected time of alignment over 50 hours.(0.6 reads/s)

    Used commands are :
    MosaikAligner -in input.dat -out aligned.dat -ia total.ref.dat -hs 15 -mm 0.05 -act 26 -m all -mhp 100 -j h.sapiens_15 -p 8

    Mosaik version : 0.9.891-linux-x64

    I will do the job with version 1.0.1367, and I want to reduce working hour...

  • #2
    In the case of Mosaik version 1.0.1367,
    aligned the lane of 119,263 '454 reads', in 246minutes (7.7 reads/s)
    to the entire human genome(3.0Gbp) using 8 processor cores....

    Comment


    • #3
      How can I create one single dat example total.ref.dat for the entire genome. The data available are in seperate files for each chromosome.
      I want to run entire index data with mosaik for all the chromosome together.
      Thanks!!

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Recent Advances in Sequencing Analysis Tools
        by seqadmin


        The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
        Today, 07:48 AM
      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin




        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
        04-22-2024, 07:01 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Today, 07:17 AM
      0 responses
      11 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-02-2024, 08:06 AM
      0 responses
      19 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-30-2024, 12:17 PM
      0 responses
      20 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-29-2024, 10:49 AM
      0 responses
      28 views
      0 likes
      Last Post seqadmin  
      Working...
      X