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  • order scaffolds

    There is a genome in unoriented and unorder scaffolds. I am interested in a specific area of the genome. Therefore I managed to align the scaffolds to a closely related species and I got an idea about the orientation of the scaffolds.

    I want to visualize it in a genome browser in the correct order.
    Any idea how to modify the orientation inside the genbank file of those scaffolds?

    Any help please`?

  • #2
    The ABACUS program was designed for this purpose and I have found it to work quite well. ABACUS is a part of the PAGIT pipeline, which includes other programs that you may find useful.

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    • #3
      Here's a duplicate question on BioStars,


      Here's an older BioPerl answer http://www.biostars.org/p/43236/

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