Header Leaderboard Ad

Collapse

Bam to UCSC readable bed file

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Bam to UCSC readable bed file

    Hi,

    Does anyone know how we can convert a bam file to u ucsc bed file in order to visualize the coverage? I am not a bioinformatician, and tried Galaxy bedtools, but after uploading the converted bed file, I couldnt see the coverage graph but all the NGS reads (huge file). Is there any other easy way to do it with or without galaxy, but without having to program anything?

    Thanks

  • #2
    You may want to use a bedgraph file if you are looking to visualize coverage. That is what you should try to create on galaxy.

    I am not sure if you are strictly looking for a utility that has a GUI interface but there are some suggestions in this biostars thread for command line usage: http://www.biostars.org/p/64495/

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Improved Targeted Sequencing: A Comprehensive Guide to Amplicon Sequencing
      by seqadmin



      Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...
      03-21-2023, 01:49 PM
    • seqadmin
      Targeted Sequencing: Choosing Between Hybridization Capture and Amplicon Sequencing
      by seqadmin




      Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...
      03-10-2023, 05:31 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 01:40 PM
    0 responses
    6 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-29-2023, 11:44 AM
    0 responses
    12 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-24-2023, 02:45 PM
    0 responses
    20 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-22-2023, 12:26 PM
    0 responses
    28 views
    0 likes
    Last Post seqadmin  
    Working...
    X