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I have some queries about split /chimeric reads: 1) Though I have read several defintions of split reads as given in this thread: http://seqanswers.com/forums/showthread.php?t=2267. I am stil unable to grasp the whole idea of split reads. can anyone please elaborate in a simple way? 2). our task is to perform SNP calling from NGS data. I used bwa-sw for mapping the roche 454 data and the mapping yielded multiple alignment for some reads. So should I remove those reads for SNP calling ? If possible, please enlighten me on above issues.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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