I have some queries about split /chimeric reads: 1) Though I have read several defintions of split reads as given in this thread: http://seqanswers.com/forums/showthread.php?t=2267. I am stil unable to grasp the whole idea of split reads. can anyone please elaborate in a simple way? 2). our task is to perform SNP calling from NGS data. I used bwa-sw for mapping the roche 454 data and the mapping yielded multiple alignment for some reads. So should I remove those reads for SNP calling ? If possible, please enlighten me on above issues.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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