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I do have a set of data containing both Exome Seq and RNA Seq. It will be interesting to see how they correlate in terms of calling. Specifically, it will be interested to see how mark duplication will affect the concordance between the RNA samples from the DNA samples. However, I do have a question: is it valid to use tools like GATK to perform snp calling on RNA Samples? To my knowledge, it seems like GATK might contain certain prior specifically designed for exome sequencing (or whole genome sequencing). Wouldn't that also affect the call concordance in the final data?
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This year’s Advances in Genome Biology and Technology (AGBT) General Meeting commemorated the 25th anniversary of the event at its original venue on Marco Island, Florida. While this year’s event didn’t include high-profile musical performances, the industry announcements and cutting-edge research still drew the attention of leading scientists.
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The biggest announcement was Roche stepping back into the sequencing platform market. In the years since...03-03-2025, 01:39 PM -
The human gut contains trillions of microorganisms that impact digestion, immune functions, and overall health1. Despite major breakthroughs, we’re only beginning to understand the full extent of the microbiome’s influence on health and disease. Advances in next-generation sequencing and spatial biology have opened new windows into this complex environment, yet many questions remain. This article highlights two recent studies exploring how diet influences microbial...02-24-2025, 06:31 AM
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