Hello everyone,
Currently, I am going deep-through our expression database (bacterial RNA-seq data) in order to get the maximum information possible. I stumbled across very simple but still very important question: From what point can we say that a gene is fairly-well expressed in terms of RPKM value? For example, if I have a RPKM value of 50, with what confidence can I say that that gene is indeed expressed, hence important for the cell in given conditions?
Would it be safe to compare to the mean expression of all genes, or perhaps compare to the expression of some HKG or even tRNA or rRNA?
Thank you in advance for your opinion,
TP
Currently, I am going deep-through our expression database (bacterial RNA-seq data) in order to get the maximum information possible. I stumbled across very simple but still very important question: From what point can we say that a gene is fairly-well expressed in terms of RPKM value? For example, if I have a RPKM value of 50, with what confidence can I say that that gene is indeed expressed, hence important for the cell in given conditions?
Would it be safe to compare to the mean expression of all genes, or perhaps compare to the expression of some HKG or even tRNA or rRNA?
Thank you in advance for your opinion,
TP
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