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  • szyszka
    Junior Member
    • Sep 2013
    • 2

    #1

    vcf troubleshoot

    I obtained a vcf file from mutect that is structured like this and is not accepted by any annotation tools like Ingenuity Variariant Analysis, etc. Can anyone please advice me on what is the problem and how can it be solved?

    #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT 52
    1 1225522 . T C . PASS SOMATIC;VT=SNP GT:AD:BQ: DP:FA:SS 0:67,2:.:68:0.029:0
    1 2372079 . G A . PASS SOMATIC;VT=SNP GT:AD:BQ: DP:FA:SS 0:9,0:.:8:0.00:0
    1 21806315 rs61775949 G C . PASS DB;SOMATIC;VT=SNP GT:AD:BQ: DP:FA:SS 0:21,0:.:21:0.00:0
    1 35649701 . G T . PASS SOMATIC;VT=SNP GT:AD:BQ: DP:FA:SS 0:44,0:.:44:0.00:0
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    One reason could be the missing set of "Meta-information lines" as specified in the VCF format: http://www.1000genomes.org/node/101

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