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  • morning latte
    Member
    • Jun 2013
    • 91

    #1

    platform for quick overview of short reads before assembly

    Hello all,

    Could you recommend any platform (server) that I can import my short reads (100bp paired-end from Illumina) before assembly or even QC to get some idea who are there? I heard that MG-RAST is one of the options. I appreciate for your answers in advance.
  • winsettz
    Member
    • Sep 2012
    • 91

    #2
    There's metaphlan and phytophlan (from the Huttenhower lab) if you're interested in taxonomy. BLASTX if you're interested in what proteins might be there.

    MG-RAST is also fairly good, and should be more robust in taxonomic calls than metaphlan and phytophlan.
    Last edited by winsettz; 10-02-2013, 01:28 PM.

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    • morning latte
      Member
      • Jun 2013
      • 91

      #3
      Thanks a lot.

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