Hi All,
I chose the length of 72 bp for RNA sequencing. But the result seems weird:
@HWI-EAS225:5:1:27:1178#0/1
GTTGTATGTACCCTAAGATTTTGNGTNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNN
+HWI-EAS225:5:1:27:1178#0/1
aaab_'aaaaaaaba_aaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB
Is that means only 20 bp sequence are correct?
What does N means?
All my sequences are similar, the first part of the sequences are normal, then NNNNNNN, then normal two nts followed by NNNNNN again. Do you think it is the problem of my sample or sequence machine?
Thanks for your explanation.
Best,
Wei
I chose the length of 72 bp for RNA sequencing. But the result seems weird:
@HWI-EAS225:5:1:27:1178#0/1
GTTGTATGTACCCTAAGATTTTGNGTNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNNNNNNNNNNN
+HWI-EAS225:5:1:27:1178#0/1
aaab_'aaaaaaaba_aaBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB
Is that means only 20 bp sequence are correct?
What does N means?
All my sequences are similar, the first part of the sequences are normal, then NNNNNNN, then normal two nts followed by NNNNNN again. Do you think it is the problem of my sample or sequence machine?
Thanks for your explanation.
Best,
Wei
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