Hi, Guys
I recently found that for most of the single exon transcripts, cufflinks seems not calculate a FPKM value for them. Has anybody notice this before? Or does anyone has a clue about how to deal with it? By the way, for 'single exon transcripts' I mean the single exon transcripts assembled by cufflinks itself. Not some RefGene.
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...07-08-2024, 03:19 PM