I have to say, I'm a little embarassed to have missed what was so clearly written in the documentation. After changing the TMP variable, HGAP ran to completion with one 4Mbase contig.
Thanks for the help.
Header Leaderboard Ad
Collapse
HGAP issue in smrtanalysis 2.0.1
Collapse
Announcement
Collapse
SEQanswers June Challenge Has Begun!
The competition has begun! We're giving away a $50 Amazon gift card to the member who answers the most questions on our site during the month. We want to encourage our community members to share their knowledge and help each other out by answering questions related to sequencing technologies, genomics, and bioinformatics. The competition is open to all members of the site, and the winner will be announced at the beginning of July. Best of luck!
For a list of the official rules, visit (https://www.seqanswers.com/forum/sit...wledge-and-win)
For a list of the official rules, visit (https://www.seqanswers.com/forum/sit...wledge-and-win)
See more
See less
X
-
Originally posted by mjhsieh View PostI think you can edit the file in analysis/etc/smrtpipe.rc and find "TMP = " entry and set it to a directory according to number four of this section:
https://github.com/PacificBioscience...em-requirement
Leave a comment:
-
Originally posted by schroedj View PostSorry for the noise, but I just found the issue.... In the log/P_Mapping/align.log file, it says "/tmp/sortg5zIfL: No space left on device". Clearly, my /tmp directory is full. Can I change the location to which Smrtanalysis saves temporary files at this point, or do I have to reconfigure the installation and set the new location during the initial configuration?
Thanks.
Leave a comment:
-
Sorry for the noise, but I just found the issue.... In the log/P_Mapping/align.log file, it says "/tmp/sortg5zIfL: No space left on device". Clearly, my /tmp directory is full. Can I change the location to which Smrtanalysis saves temporary files at this point, or do I have to reconfigure the installation and set the new location during the initial configuration?
Thanks.
Leave a comment:
-
HGAP issue in smrtanalysis 2.0.1
I'm trying to assemble a bacterial genome (about 4.1Mbases in length) using HGAP in smrtanalysis 2.0.1. The job runs until the draft assembly is finished and then fails after that point. I am at a loss as to how to fix this problem. I am running this on a single node machine. I have attached the master.log and smrtpipe.log files. I would really appreciate any help I can get with this matter.
I should mention that I've run HGAP on the included lambda SMRT cell and it does get all the way to a polished genome, so it seems to me that I have smrtanalysis set up correctly on my system.
Latest Articles
Collapse
-
by seqadmin
Developments in sequencing technologies and methodologies have transformed the field of epigenetics, giving researchers a better way to understand the complex world of gene regulation and heritable modifications. This article explores some of the diverse sequencing methods employed in the study of epigenetics, ranging from classic techniques to cutting-edge innovations while providing a brief overview of their processes, applications, and advances.
Methylation Detect...-
Channel: Articles
Yesterday, 10:46 AM -
-
Differential Expression and Data Visualization: Recommended Tools for Next-Level Sequencing Analysisby seqadmin
After covering QC and alignment tools in the first segment and variant analysis and genome assembly in the second segment, we’re wrapping up with a discussion about tools for differential gene expression analysis and data visualization. In this article, we include recommendations from the following experts: Dr. Mark Ziemann, Senior Lecturer in Biotechnology and Bioinformatics, Deakin University; Dr. Medhat Mahmoud Postdoctoral Research Fellow at Baylor College of Medicine;...-
Channel: Articles
05-23-2023, 12:26 PM -
-
by seqadmin
Continuing from our previous article, we share variant analysis and genome assembly tools recommended by our experts Dr. Medhat Mahmoud, Postdoctoral Research Fellow at Baylor College of Medicine, and Dr. Ming "Tommy" Tang, Director of Computational Biology at Immunitas and author of From Cell Line to Command Line.
Variant detection and analysis tools
Mahmoud classifies variant detection work into two main groups: short variants (<50...-
Channel: Articles
05-19-2023, 10:03 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Deep Sequencing Unearths Novel Genetic Variants: Enhancing Precision Medicine for Vascular Anomalies
by seqadmin
Started by seqadmin, Today, 07:33 AM
|
0 responses
7 views
0 likes
|
Last Post
by seqadmin
Today, 07:33 AM
|
||
Unveiling Genetic Associations Through Transcription Factor Binding Quantitative Trait Loci
by seqadmin
Started by seqadmin, Yesterday, 07:50 AM
|
0 responses
4 views
0 likes
|
Last Post
by seqadmin
Yesterday, 07:50 AM
|
||
Exploring French-Canadian Ancestry: Insights into Migration, Settlement Patterns, and Genetic Structure
by seqadmin
Started by seqadmin, 05-26-2023, 09:22 AM
|
0 responses
8 views
0 likes
|
Last Post
by seqadmin
05-26-2023, 09:22 AM
|
||
Started by seqadmin, 05-24-2023, 09:49 AM
|
0 responses
20 views
0 likes
|
Last Post
by seqadmin
05-24-2023, 09:49 AM
|
Leave a comment: