Header Leaderboard Ad

Collapse

Renaming fasta headers - string within a string issue

Collapse

Announcement

Collapse

SEQanswers June Challenge Has Begun!

The competition has begun! We're giving away a $50 Amazon gift card to the member who answers the most questions on our site during the month. We want to encourage our community members to share their knowledge and help each other out by answering questions related to sequencing technologies, genomics, and bioinformatics. The competition is open to all members of the site, and the winner will be announced at the beginning of July. Best of luck!

For a list of the official rules, visit (https://www.seqanswers.com/forum/sit...wledge-and-win)
See more
See less
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Renaming fasta headers - string within a string issue

    Hi Everyone,

    I am in the midst of renaming some fasta headers to include the chromosomes they have been mapped to (from a tab delimited txt file). I don't have any problems setting up the hash for the scaffold and chromosome locations...the problem is that I have some short strings contained in longer strings (scaffold1 and scaffold10 and scaffold100). Usually I would add "$" to the end of my search term (/scaffold1$/) and thereby indicate the end of the search string, but I'm not sure how to make use of this when the search term is a variable ($hash{$scaff/$/}). Advice?

    Below is my script so far...and TIA!

    Code:
    #!/bin/bash/perl
    #mod-header2include-chrom.pl
    #This script is intended to read in a fasta file and a tab-delimited file and use the information from the tab-delimited file to modify the header.
    #In this case, we are appending to the header (e.g. "scaffold671") the chromosome to which it has been mapped and the number of genes on this scaffold.
    
    use strict;
    use warnings;
    
    open (DATA, "<genome-assoc-chromosomes.txt") or die "Could not open genome chromosome mapping data: $!\n";
    open (FASTA, "<scafSeq.FG.fill") or die "Could not open Fasta file: $!\n";
    
    my %hash;
    
    while (<DATA>)
    {
    chomp;
    if ($_ =~ "scafold"){ #skip header - scafold is spelled incorrectly on purpose;
    next;
    }
    else {
    my ($key, $chrom, $len, $gene) = split /\t/;
    $hash{$key} = $chrom;
    }
    }
    
    while (<FASTA>){
    
    my $line = $_;
    chomp ($line);
    if ( defined $hash{$line} ) {
    print "$line-$hash{$line}";
    }
    else { print $line; }
    
    }

  • #2
    Hi,
    Kindly upload a sample of both files (scafSeq.FG.fill and genome-assoc-chromosomes.txt). Without the fasta file, I am wondering how the following:
    while (<FASTA>){
    my $line = $_;
    chomp ($line);
    if ( defined $hash{$line} ) {


    reads in the header line with ">" from "<scafSeq.FG.fill"

    does the variable $key in "$hash{$key} = $chrom;" contain ">"?

    Comment

    Latest Articles

    Collapse

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 08:56 PM
    0 responses
    8 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, Yesterday, 07:33 AM
    0 responses
    8 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 05-31-2023, 07:50 AM
    0 responses
    4 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 05-26-2023, 09:22 AM
    0 responses
    9 views
    0 likes
    Last Post seqadmin  
    Working...
    X