Hi all
What is the best approach when I want to get expression estimates from RNA-seq data for a species where the full genome does not exist but UniGene has a lot of sequences for it? In my case it is wheat.
Is it more appropriate to map the reads against the unique sequences in UniGene or to map them against all sequences?
Or is there a completely different approach?
regards
hubert
What is the best approach when I want to get expression estimates from RNA-seq data for a species where the full genome does not exist but UniGene has a lot of sequences for it? In my case it is wheat.
Is it more appropriate to map the reads against the unique sequences in UniGene or to map them against all sequences?
Or is there a completely different approach?
regards
hubert
Comment