Hi all,
I just have a quick question concerning Illumina RNAseq. If you have a gene that gets about 1 read in 7 biological replicates, would you consider this gene to be expressed or not expressed? What is the minimal number of reads (or normalized expression) that you would consider it expressed? Are there any general rules I can orientate myself on?
Thanks,
Lukas
I just have a quick question concerning Illumina RNAseq. If you have a gene that gets about 1 read in 7 biological replicates, would you consider this gene to be expressed or not expressed? What is the minimal number of reads (or normalized expression) that you would consider it expressed? Are there any general rules I can orientate myself on?
Thanks,
Lukas
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