Hello i am trying to use GEM for first time but i come across some problems and i wonder if someone can give me a solution.
I try to index with the command
gem-indexer -T 4 -i genome.fa -o hg19
and i get this error
Creating sequence and location files... done.
Computing DNA BWT (likely to take long)... done.
Generating index (likely to take long)...Fatal error: exception Failure("Command 'echo "-------gem-indexer_generate" >> hg19.log && gem-indexer_generate --append -i hg19.fmi_dna-cont -o hg19.gem -s 32 --index_type fmi-dna -b hg19.fmi_dna-bwt --mm-tmp-prefix "/tmp/mm_new-" >> hg19.log 2>&1' could not be run successfull
when i try to map with the output file
gem-mapper -q ignore -I hg19.gem -i SRR502337.fastq -o SRR502337_gem
i get this error
Tue Nov 12 12:37:54 2013 -- Loading index (likely to take long)...Fatal error (setup.c:167,input_gem_header)
Bad GEM header
any suggestions?
I try to index with the command
gem-indexer -T 4 -i genome.fa -o hg19
and i get this error
Creating sequence and location files... done.
Computing DNA BWT (likely to take long)... done.
Generating index (likely to take long)...Fatal error: exception Failure("Command 'echo "-------gem-indexer_generate" >> hg19.log && gem-indexer_generate --append -i hg19.fmi_dna-cont -o hg19.gem -s 32 --index_type fmi-dna -b hg19.fmi_dna-bwt --mm-tmp-prefix "/tmp/mm_new-" >> hg19.log 2>&1' could not be run successfull
when i try to map with the output file
gem-mapper -q ignore -I hg19.gem -i SRR502337.fastq -o SRR502337_gem
i get this error
Tue Nov 12 12:37:54 2013 -- Loading index (likely to take long)...Fatal error (setup.c:167,input_gem_header)
Bad GEM header
any suggestions?