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  • efthimis
    Junior Member
    • Mar 2013
    • 1

    #1

    Gem index and mapping

    Hello i am trying to use GEM for first time but i come across some problems and i wonder if someone can give me a solution.
    I try to index with the command

    gem-indexer -T 4 -i genome.fa -o hg19

    and i get this error

    Creating sequence and location files... done.
    Computing DNA BWT (likely to take long)... done.
    Generating index (likely to take long)...Fatal error: exception Failure("Command 'echo "-------gem-indexer_generate" >> hg19.log && gem-indexer_generate --append -i hg19.fmi_dna-cont -o hg19.gem -s 32 --index_type fmi-dna -b hg19.fmi_dna-bwt --mm-tmp-prefix "/tmp/mm_new-" >> hg19.log 2>&1' could not be run successfull


    when i try to map with the output file

    gem-mapper -q ignore -I hg19.gem -i SRR502337.fastq -o SRR502337_gem

    i get this error
    Tue Nov 12 12:37:54 2013 -- Loading index (likely to take long)...Fatal error (setup.c:167,input_gem_header)
    Bad GEM header

    any suggestions?
    Last edited by efthimis; 11-13-2013, 02:30 AM.

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