Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • efthimis
    Junior Member
    • Mar 2013
    • 1

    #1

    Gem index and mapping

    Hello i am trying to use GEM for first time but i come across some problems and i wonder if someone can give me a solution.
    I try to index with the command

    gem-indexer -T 4 -i genome.fa -o hg19

    and i get this error

    Creating sequence and location files... done.
    Computing DNA BWT (likely to take long)... done.
    Generating index (likely to take long)...Fatal error: exception Failure("Command 'echo "-------gem-indexer_generate" >> hg19.log && gem-indexer_generate --append -i hg19.fmi_dna-cont -o hg19.gem -s 32 --index_type fmi-dna -b hg19.fmi_dna-bwt --mm-tmp-prefix "/tmp/mm_new-" >> hg19.log 2>&1' could not be run successfull


    when i try to map with the output file

    gem-mapper -q ignore -I hg19.gem -i SRR502337.fastq -o SRR502337_gem

    i get this error
    Tue Nov 12 12:37:54 2013 -- Loading index (likely to take long)...Fatal error (setup.c:167,input_gem_header)
    Bad GEM header

    any suggestions?
    Last edited by efthimis; 11-13-2013, 02:30 AM.

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
19 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
26 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-13-2026, 09:56 AM
0 responses
22 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-09-2026, 11:14 AM
0 responses
28 views
0 reactions
Last Post SEQadmin2  
Working...