Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • DrS
    Member
    • Mar 2013
    • 17

    #1

    Mapping HiSeq to Proteins

    I've got a project with a tight deadline that will include needing to map some RNASeq data to a reference.

    There is a reference transcriptome "published", but only the protein sequences are available, not the DNA.

    Does anyone know of any tools that will let me map my fastq files to a protein reference? I've been searching around, but might get an answer faster this way

    Right now I'm thinking (dreading?) I may need to do a fastq-to-fasta and use exonerate.

    Thanks for any ideas.
  • anth
    Member
    • Jul 2011
    • 18

    #2
    Why not perform a de novo assembly with your RNAseq data instead?

    Comment

    • DrS
      Member
      • Mar 2013
      • 17

      #3
      That is running but will likely take a couple of days, which is part of why I was looking for an alternative approach.

      Plus now I'm simply curious - is there a tool out there that will let you make RNASeq reads to a proteome?

      Comment

      • Richard Finney
        Senior Member
        • Feb 2009
        • 701

        #4
        Check out blat.
        Blast can do it , too.

        *at least I think they do*

        Comment

        Latest Articles

        Collapse

        • kinpict.com
          Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
          by kinpict.com
          The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
          Today, 06:37 PM
        • SEQadmin2
          16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
          by SEQadmin2





          NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
          10-30-2026, 06:46 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, 11-12-2026, 07:59 AM
        0 responses
        28 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 11-05-2026, 09:25 AM
        0 responses
        35 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 11-03-2026, 11:54 AM
        2 responses
        56 views
        0 reactions
        Last Post kinpict.com  
        Started by SEQadmin2, 10-28-2026, 09:57 AM
        0 responses
        175 views
        0 reactions
        Last Post SEQadmin2  
        Working...