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  • DrS
    Member
    • Mar 2013
    • 17

    #1

    Mapping HiSeq to Proteins

    I've got a project with a tight deadline that will include needing to map some RNASeq data to a reference.

    There is a reference transcriptome "published", but only the protein sequences are available, not the DNA.

    Does anyone know of any tools that will let me map my fastq files to a protein reference? I've been searching around, but might get an answer faster this way

    Right now I'm thinking (dreading?) I may need to do a fastq-to-fasta and use exonerate.

    Thanks for any ideas.
  • anth
    Member
    • Jul 2011
    • 18

    #2
    Why not perform a de novo assembly with your RNAseq data instead?

    Comment

    • DrS
      Member
      • Mar 2013
      • 17

      #3
      That is running but will likely take a couple of days, which is part of why I was looking for an alternative approach.

      Plus now I'm simply curious - is there a tool out there that will let you make RNASeq reads to a proteome?

      Comment

      • Richard Finney
        Senior Member
        • Feb 2009
        • 701

        #4
        Check out blat.
        Blast can do it , too.

        *at least I think they do*

        Comment

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