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I am doing some analysis using XHMM. I was adviced to use a value of 12 as the mean number of targets. Being a recent convert to bioinformatics, I am a bit confused about what a target is? The output gives a variable called NUM_TARG. Is target in XHMM the same as markers in VarScan2? OR does a target correspond to 1 EXON? Does it have any relationship to the mean number of targets. I would really appreciate if some one who has used XHMM could enlighten me a bit.
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM