I'm trying to extract sequences from a reference based assembly (the reference is a transcriptome) based on coverage and length in CLC. So, I want to extract the sequences with >5x coverage and >200bp. This is simple enough for de novo assembly (extract subset), is there something like this for ref-based?
-
-
When you run "map reads to reference" you should selected the option "Create stand-alone read mappings" (in the last wizard step)
The output will be table that is very similar as the the novo output and you can extract a subset. -
Hi
I have a similar problem but with contigs ,I want to extract sequences between the contigs when the contigs are aligned to a refrence..how do I extract these sequences..Comment
-
CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
Comment