Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • horvathdp
    Member
    • Dec 2011
    • 66

    more problems with RSEM in Trinity build

    I have down-loaded , and built Trinity, and am still struggling to get my downstream analyses to run.

    here you can see I've ls'd the RSEM file to ensure the executables were present, but they are not found when I try running the program.

    Any thoughts as to why?

    horvathdp@128:/vol-8A5507EB/Trinity/trinityrnaseq_r20131110/util/RSEM_util$ ls
    merge_RSEM_counts_and_labels_single_table.pl Nx_rsem_to_table.pl
    merge_RSEM_frag_counts_single_table.pl run_RSEM_align_n_estimate.pl
    horvathdp@128:/vol-8A5507EB/Trinity/trinityrnaseq_r20131110/util/RSEM_util$ run_RSEM_align_n_estimate.pl --transcripts paradormancy_Trinity_contigs.fasta \ --seqType fq --left 0Hr_1_R1.fq --right 0Hr_1_R2.fq
    bash: run_RSEM_align_n_estimate.pl: command not found

Latest Articles

Collapse

  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM
  • SEQadmin2
    From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
    by SEQadmin2


    Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
    ...
    06-02-2026, 10:05 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 06-26-2026, 11:10 AM
0 responses
15 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-17-2026, 06:09 AM
0 responses
48 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-09-2026, 11:58 AM
0 responses
107 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-05-2026, 10:09 AM
0 responses
125 views
0 reactions
Last Post SEQadmin2  
Working...