I know this for nextgen but can anyone tell me the best program to assemble paired-end sanger reads.
regards
Brian
regards
Brian
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Good-old Phred/phrap? http://phrap.org/ -
There are several options...
Hi,
I would recommed (in this order):
ARACHNE v3.2 (broad institute)
PHUSION (Sanger institute)
PCAP (Iowa State University)
CA-BOG (Celera Assembler)
Check their sites. Some of them are easier to install and use, and to be honest depending on your genome, one gives better results than the others.
Cheers.Comment
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