Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Problems with samtools-0.19.0 (MingW)

    I got the program to compile without error in MingW, simply by adding a few words to the Makefile and hitting Make:
    • Iwin32 to INCLUDES
    • -Lwin32 -L. -lbam -lm -lz -lpthread -lws2_32 to samtools:lib-recur cmd


    Samtools 0.18.0 compiles perfectly like this (although on some systems you have to substitute "lpdcurses" with "lcurses")

    However, although samtools 0.19.0 works fine in some limited capacity, it has some problems. For instance, on the example:
    samtools faidx examples/ex1.fa
    samtools import examples/ex1.fa.fai examples/ex1.sam.gz ex1.bam
    samtools index ex1.bam

    It fails on this last line with
    "[bam_index_core] truncated file? Continue anyway. (-2)"
    Is this a problem with the indexer (i.e. the third command), or a problem with the index itself (i.e. the "import" command)?

    [edit]
    Solved. That was dumb of me. Just start with a clean MinGW instead of using someone else's.
    Last edited by Seqwince; 11-24-2013, 07:48 PM.

  • #2
    FYI, "samtools import" has been deprecated for a while. Use "samtools view -Sbo ex1.bam examples/ex1.sam" (unless you actually do need to reheader the BAM file, in which case there's the -t option). At the moment, the "import" command just runs "view", but I wouldn't be surprised if that gets dropped eventually (not to mention that "view" is more flexible).

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM
    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Today, 11:49 AM
    0 responses
    10 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, Yesterday, 08:47 AM
    0 responses
    16 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    61 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    60 views
    0 likes
    Last Post seqadmin  
    Working...
    X