I'm working with some Reduced Representation Bisulfite Sequenced datasets. After aligning the reads to the reference genome (Sus Scrofa), I want to determine the number (percent) of CpG islands I have covered in my dataset (classified as minimum 200bp, >50% GC content), but I'm having trouble figuring out the best way to do this. Does anyone have any experience with this type of analysis? Any help would be appreciated. Thank you very much!
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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