I'm working with some Reduced Representation Bisulfite Sequenced datasets. After aligning the reads to the reference genome (Sus Scrofa), I want to determine the number (percent) of CpG islands I have covered in my dataset (classified as minimum 200bp, >50% GC content), but I'm having trouble figuring out the best way to do this. Does anyone have any experience with this type of analysis? Any help would be appreciated. Thank you very much!
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM