Joined a workshop recently. They are talking about the RNA Seq which is not my territory. Lack of knowledge on this field.
So any diff between those two? Thanks
So any diff between those two? Thanks
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Splice aware aligners take into account the splice junctions between exons in gene regions while aligning RNA-seq reads. So if a read spans the splice junction on either side, it gets aligned as such.
In ungapped alignment, the splice junction would indicate only a partial match for the reads that span these junctions and it might not be aligned.
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